Whole genome sequencing of E. yunnanense - SRA

SRX3406417: Whole genome sequencing of E. yunnanense
1 ILLUMINA (HiSeq X Ten) run: 33.8M spots, 10.1G bases, 4.3Gb downloads

Design: To generate the genomic data for E. heterophyllum and E. yunnanense, we used the CTAB method to extract high-quality genomic DNA from 5g of leaf tissues for each species. The quality of the high molecular weight DNA was checked by 1% agarose gel electrophoresis. Around 200 ¦Ìg of genomic DNA was used for library construction. We extracted total RNA for each tissue collected from both species (i.e. leaves, flowers and roots for E. heterophyllum, and leaves, leafstalks and roots for E. yunnanense). The quality of the extracted RNA was verified on a 1% agarose gel. DNase treatment was performed before library construction. For each sample, 5 ¦Ìg of the total high-quality RNA was used for sequencing.

Submitted by: Sichuan University

Study: The genomes of two Eutrema species

PRJNA419026 • SRP125248 • All experiments • All runs

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To investigate the high-altitude adaptation in plants, we generated genomic data for two diploid perennial species of the Eutrema genus (Brassicaceae). The two genomes reported here constitute important genetic resources for diverse studies.

Sample:

SAMN08040565 • SRS2699520 • All experiments • All runs

Organism: Eutrema yunnanense

Library:

Name: Eyu_800bp

Instrument: HiSeq X Ten

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: PAIRED

Runs: 1 run, 33.8M spots, 10.1G bases, 4.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR6306020	33,817,333	10.1G	4.3Gb	2018-02-22

ID:: 4747423

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